| $150,000–350,000 | MARK R. FINKLER, D.V.M., P.C. | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | MARTINSBURG OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $1–2 million | MATTERN & CRAIG INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | MB CONTRACTORS, INC. | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | MCCLEOD ENTERPRISES, LLP | ROANOKE, VA | HomeTrust Bank |
| $150,000–350,000 | METIS SERVICES, INC. | ROANOKE, VA | Village Bank |
| $150,000–350,000 | MID-ATLANTIC HEATING & COOLING, INC. | ROANOKE, VA | Freedom First FCU |
| $2–5 million | MKG ENTERPRISES LLC | ROANOKE, VA | Truist Bank |
| $150,000–350,000 | MONTANO'S INTERNATIONAL GOURMET SHOP'S INC. | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | MORCOM, INC. | ROANOKE, VA | Skyline National Bank |
| $350,000–1 million | MORGANTOWN OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | MOSS & ROCOVICH, ATTORNEYS-AT-LAW, P.C. | ROANOKE, VA | Truist Bank |
| $350,000–1 million | MOUNTAIN ROOFING INC. | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | MOUNTAIN VIEW LAWN CARE LLC | ROANOKE, VA | Truist Bank |
| $350,000–1 million | MURFREESBORO OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | MURRAY E. JOINER JR MD PC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | NATIONAL O&M INC | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | NATIONAL POOLS OF ROANOKE INC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | NETWORK COMPUTING GROUP, INC. | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | NEW HOPE SUPPORT SERVICES, LLC | ROANOKE, VA | Bank of Botetourt |
| $350,000–1 million | NICUS SOFTWARE, INC. | ROANOKE, VA | Pinnacle Bank |
| $1–2 million | NORTH CROSS SCHOOL | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | NORTH ROANOKE ASSISTED LIVING LLC | ROANOKE, VA | First Bank |
| $350,000–1 million | NORTHWEST HARDWARE COMPANY, INCORPORATED | ROANOKE, VA | Truist Bank |
| $2–5 million | ONCOLOGY & HEMATOLOGY ASSOCIATES OF SOUTHWEST VIRGINIA, INC. | ROANOKE, VA | Bank of Botetourt |
| $350,000–1 million | OPN LAW | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $2–5 million | OPTICAL CABLE CORPORATION | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | ORCHARD HILLS CHURCH | ROANOKE, VA | Freedom First FCU |
| $150,000–350,000 | PARKER DESIGN GROUP, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | PARKWAY WESLEYAN CHURCH INC | ROANOKE, VA | Truist Bank |
| $150,000–350,000 | PENG CHENG, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | PENINSULA PATHOLOGY ASSOCIATES INC | ROANOKE, VA | TowneBank |
| $350,000–1 million | PHYSICIAN ASSOCIATES OF VIRGINIA PC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | PHYSICIANS CARE OF VIRGINIA, P.C. | ROANOKE, VA | Skyline National Bank |
| $350,000–1 million | PHYSICIANS TO CHILDREN, INC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | PHYSICIANS TO WOMEN, INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | PITMAN CONSTRUCTION COMPANY INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | PLASTIC FABRICATING, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | PODELL INC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | POTOMAC PLACE ASSOCIATES | ROANOKE, VA | Wells Fargo Bank, National Association |
| $150,000–350,000 | POWER LINE RENT EQUIPMENT INC | ROANOKE, VA | Truist Bank |
| $350,000–1 million | PR OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | PRECISION STEEL MANUFACTURING CORPORATION | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | PRIORITY AUTOMOTIVE ROANOKE, INC. | ROANOKE, VA | TowneBank |
| $350,000–1 million | PRMC OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | PROFESSIONAL EDUCATION AND RESEARCH INSTITUTE, INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | PROFESSIONAL NETWORK SERVICES INC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | PROPERTY MANAGEMENT OF ROANOKE LLC | ROANOKE, VA | United Bank |
| $150,000–350,000 | PSYCHOLOGICAL HEALTH ROANOKE, PC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | QUALITY COFFEE SERVICE | ROANOKE, VA | Truist Bank |
| $150,000–350,000 | QUICK FIX REAL ESTATE LLC | ROANOKE, VA | Freedom First FCU |
| $1–2 million | R & K SOLUTIONS, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | R FRALIN CONSTRUCTION INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | RADIOLOGY ASSOCIATES OF ROANOKE, PC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | RAINWATER MANAGEMENT SOLUTIONS INC. | ROANOKE, VA | Atlantic Union Bank |
| $1–2 million | RANGER FUEL COAL CORPORATION | ROANOKE, VA | MVB Bank, Inc. |
| $350,000–1 million | RICHARD D. SMITH II, D.D.S., P.C. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $1–2 million | RISK MANAGEMENT PROGRAMS, INC. | ROANOKE, VA | Village Bank |
| $150,000–350,000 | ROANOKE AIRPORT HOTEL PARTNERS LLC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | ROANOKE AIRPORT TRANSPORTATION SERVICE, INC. | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | ROANOKE CATHOLIC SCHOOL | ROANOKE, VA | SouthState Bank, National Association |
| $150,000–350,000 | ROANOKE COOPERATIVE ASSOCIATION, LTD. | ROANOKE, VA | Freedom First FCU |
| $150,000–350,000 | ROANOKE FRUIT AND PRODUCE COMPANY | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | ROANOKE GLASS INCORPORATED | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | ROANOKE IMPORTS, INC. | ROANOKE, VA | Truist Bank |
| $350,000–1 million | ROANOKE ORAL SURGERY INC. | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | ROANOKE STAMP & SEAL CO. | ROANOKE, VA | First Bank |
| $150,000–350,000 | ROANOKE SYMPHONY ORCHESTRA | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | ROANOKE VALLEY PIZZA, INC. | ROANOKE, VA | City National Bank of Florida |
| $150,000–350,000 | ROANOKE VALLEY SOCIETY FOR THE PREVENTION OF CRUELTY TO ANIM | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | ROBERT YOUNG'S AUTO & TRUCK, INC. | ROANOKE, VA | First Bank |
| $1–2 million | ROCKYDALE QUARRIES CORP | ROANOKE, VA | Bank of America, National Association |
| $150,000–350,000 | RUBICON PLANNING, LLC | ROANOKE, VA | Atlantic Union Bank |
| $1–2 million | RWS ENTERPRISES, INC. | ROANOKE, VA | Truist Bank |
| $350,000–1 million | S LEWIS LIONBERGER CONSTRUCTION COMPANY | ROANOKE, VA | Freedom First FCU |
| $350,000–1 million | S. C. ROSSI & COMPANY, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | SAFEHOUSE SIGNS, INC | ROANOKE, VA | Bank of the James |
| $150,000–350,000 | SCHROEDER OPTICAL CO INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | SECOND PRESBYTERIAN CHURCH FUND, INC. | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | SECURITEC SCREENING SOLUTIONS, INC. | ROANOKE, VA | Truist Bank |
| $150,000–350,000 | SECURITY LOCK & KEY INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | SECURITY SCALE SERVICE INC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | SENTINEL PROPERTIES, INC. | ROANOKE, VA | The National Bank of Blacksburg |
| $1–2 million | SFCS INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | SHIVELY ELECTRICAL CO INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | SJ CONNER & SONS INC | ROANOKE, VA | Customers Bank |
| $350,000–1 million | SMITH PACKETT MED-COM LLC | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | SOUTHERN REFRIGERATOR CORPORATION | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | SOUTHERN STATE ELECTRIC AND PLUMBING | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | SPECTRUM DESIGN, PC | ROANOKE, VA | Freedom First FCU |
| $350,000–1 million | ST VINCENT'S HOME | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | ST. JOHN'S EPISCOPAL CHURCH | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | STAFFORD OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | STAGE SOUND INC | ROANOKE, VA | Wells Fargo Bank, National Association |
| $1–2 million | STAR CITY COMICS & GAMES, INC. | ROANOKE, VA | First Bank |
| $150,000–350,000 | STATE COLLEGE OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | STEEL TRADERS SHIPPING, LTD. | ROANOKE, VA | Bank of Botetourt |
| $150,000–350,000 | STONER QUALITY WATER, INC | ROANOKE, VA | Manufacturers and Traders Trust Company |
| $150,000–350,000 | TANGLEWOOD HOSPITALITY INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | TAUBMAN MUSEUM OF ART | ROANOKE, VA | Atlantic Union Bank |