| $150,000–350,000 | THE HOLLINS GROUP, INC. | ROANOKE, VA | First Bank |
| $150,000–350,000 | THE ROANOKER RESTAURANT, INC. | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | THE WESTERN VIRGINIA FOUNDATION FOR THE ARTS AND SCIENCES | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | TOMLIN HAIR CARE OF VIRGINIA SC, INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | TOMLIN HAIR CARE, INC. | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | TRANSFORMED LEADERSHIP LLC | ROANOKE, VA | Select Bank |
| $350,000–1 million | TRAYMARK, LLC | ROANOKE, VA | Atlantic Union Bank |
| $1–2 million | TREAD CORPORATION | ROANOKE, VA | Freedom First FCU |
| $350,000–1 million | UNIFIED HUMAN SERVICES TRANSPORTATION SYSTEMS, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | UNITED SUPPORT SERVICES, LLC. | ROANOKE, VA | Bank of Botetourt |
| $150,000–350,000 | UNITED WAY OF ROANOKE VALLEY INC. | ROANOKE, VA | Atlantic Union Bank |
| $1–2 million | VA LUTHERAN HOMES INC | ROANOKE, VA | Atlantic Union Bank |
| $2–5 million | VALCOM, INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | VALLEY BOILER & MECHANICAL INC. | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | VALLEY MACHINE CO INC | ROANOKE, VA | Truist Bank |
| $150,000–350,000 | VALLEY WEALTH GROUP, INC. | ROANOKE, VA | Bank of Botetourt |
| $2–5 million | VARNEY, INC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | VARSITY LANDSCAPING & GROUNDS LLC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | VIRGINIA AUTISM AND BEHAVIOR CONSULTING LLC | ROANOKE, VA | Freedom First FCU |
| $350,000–1 million | VIRGINIA BEACH OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | VIRGINIA BUILDING SERVICES OF ROANOKE INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | VIRGINIA INTERVENTIONAL PAIN & SPINE CENTER INC. | ROANOKE, VA | Atlantic Union Bank |
| $2–5 million | VIRGINIA TRUCK CENTER, INC. | ROANOKE, VA | Pinnacle Bank |
| $2–5 million | VISTAR EYE CENTER INC | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | W. C. BUTLER HEATING AND AIR CONDITIONING INC. | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $1–2 million | W. S. CONNELLY & CO., INC. | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | WAGNER FOOD EQUIPMENT INC | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | WALKER MACHINE AND FOUNDRY CORP | ROANOKE, VA | Wells Fargo Bank, National Association |
| $150,000–350,000 | WATER CHEMISTRY INCORPORATED | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $350,000–1 million | WEBB'S OIL COMPANY INCORPORATED | ROANOKE, VA | First-Citizens Bank & Trust Company |
| $150,000–350,000 | WEED CONTROL, INC. | ROANOKE, VA | The First Bank and Trust Company |
| $1–2 million | WENDY'S OF WESTERN VIRGINIA, INC. | ROANOKE, VA | City National Bank |
| $350,000–1 million | WEST ASHLEY OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | WESTERN SIZZLIN FRANCHISE CORP | ROANOKE, VA | Wells Fargo Bank, National Association |
| $350,000–1 million | WESTERN SIZZLIN STORES INC | ROANOKE, VA | Wells Fargo Bank, National Association |
| $150,000–350,000 | WESTERN VIRGINIA EMERGENCY MEDICAL SERVICES COUNCIL, INC. | ROANOKE, VA | Pinnacle Bank |
| $150,000–350,000 | WINGATE AND ASSOCIATES LTD | ROANOKE, VA | Pinnacle Bank |
| $2–5 million | WOODS ROGERS PLC | ROANOKE, VA | Atlantic Union Bank |
| $350,000–1 million | YORK OPERATIONS, LLC | ROANOKE, VA | Pinnacle Bank |
| $350,000–1 million | YOUNG MEN'S CHRISTIAN ASSOCIATION OF VIRGINIA'S BLUE RIDGE I | ROANOKE, VA | Atlantic Union Bank |
| $150,000–350,000 | ZIIVA INC. | ROANOKE, VA | Truist Bank |